Embrapa - Empresa Brasileira de Pesquisa Agropecuária;Fundação Universidade de Brasília
发明人:
Carlos Bloch Júnior,Genaro Ribeiro de Paiva,José Roberto de Souza de Almeida Leite,Luciano Paulino da Silva
申请号：
BR0104510
公开号：
BRPI0104510B1
申请日:
2001.07.27
申请国别(地区):
BR
年份:
2017
代理人:
摘要：
"antibiotic peptides with broad spectrum antimicrobial activity". This invention relates to antimicrobial peptides having the same amino acid sequence and at least the same antimicrobial activity as those presented by phylloseptins which are isolated from the skin of phyllomedusa hypochondrialis. The peptides have no homology with other known peptides. 19 amino acid antimicrobial peptides are cationic and based on their primary structure all conform to the Î ± -amphiphilic helix pattern. peptide masses determined by spectrometry are in the range of 1.9 to 2.0 kda. The amphiphilic nature of these peptides presumably outlines their biological activities that enable them to associate with lipid membranes and disrupt normal membrane functions. however, no hemolytic activity was observed for these peptides, suggesting a selectivity for prokaryotic over eukaryotic membranes. These peptides may be used in compositions to retard plant pathogens and to protect plants from pathogens, comprising at least one antibiotic peptide and an agronomically acceptable carrier. Another embodiment of the invention is to provide therapeutic compositions suitable for human, veterinary or pharmaceutical use comprising one or more peptides disclosed in this invention and a suitable pharmacological carrier."peptídeos antibióticos com atividade antimicrobiana de amplo espectro". esta invenção refere-se a peptídeos antimicrobianos, tendo a mesma seqüência de aminoácidos e pelo menos a mesma atividade antimicrobiana das apresentadas pelas phylloseptinas que são isoladas da pele de phyllomedusa hypochondrialis. os peptídeos não têm nenhuma homologia com outros peptídeos conhecidos. os peptídeos antimicrobianos de 19 aminoácidos são catiônicos e, baseado em sua estrutura primária, todos se ajustam ao padrão de hélice <；244>；-anfifílica. as massas dos peptídeos, determinadas por espectrometria estão na faixa de 1.9 to 2.0 kda. a natureza anfifílica desses peptídeos, presumivelmente delineia suas
