PIONEER HI-BRED INTERNATIONAL, INC.;E.I. DUPONT DE NEMOURS AND COMPANY
发明人:
申请号：
ARP150102972
公开号：
AR101898A1
申请日:
2015.09.16
申请国别(地区):
AR
年份:
2017
代理人:
摘要：
Methods and compositions are provided that use a Silencing element that, when ingested by a plague, as a plague of Coleoptera Pest Diabrotica plants or plants, reduces the expression of a Target sequence in the plague. Objective to describe various polynucleotides exposed in any of the sec. With no ident.Described in the present description (but not including the First Direct and inverse) or variants or Fragments thereof or bathroom, where a reduction in the expression of one or more Sequences in the target Pest Pest Controls (i.e., has insectic activity IDA).In addition, we provide plants, Parts of plants, bacteria, and other host cells comprising the elements of silencing, or Fragments or variants of these supplements.Claim 1: an RNA Construct comprising at least one region of double stranded rna, which at least comprises a Chain polinucle u00f3tido complementary to a Nucleotide sequence that encodes a Protein comprising at least 45% amino acid identity with the Protein Encoded by the sec. With no. Ident.1 or 71 or Fragments or variants of these, or bathroom these； where the polinucle u00f3tido encodes a Silencing element that has insecticidal activity against an insect Pest of plants； and where the RNA Construct has a lt u2085 u2080 less than 80% of the average lt u2085 u2080, Where the lt u2085 u2080 is determined with the use of the sec. With no ident.: 5, 7 and 9, or where lt u2085 u2080 average is determined with the use of a Nucleotide sequence that encodes a protein homologous comprising at least 45% amino acid identity with the Protein as coded by each of the sec. With no. Ident.: 5, 7, and 9； where the lt u2085 u2080 is determined with the use of a test lt u2085 u2080 larvae / neonates that comprises a First feed Stage, where it feeds on Insect larvae during 24 hours with an artificial Diet for insects that do not understand the Construct of RNA Test； and a Second Phase of foodWhere it feeds on Insect larvae during the Eleven days with an artificial Diet
