The present invention relates to biocide zinc oxide microparticles, preparation method and use thereof. More particularly, the present invention relates to zinc oxide microparticles comprising platelets, wherein the platelets have an edge length less between 30 nm and 200 nm, are stacked in direct contact with each other along the stacking c-axis by means of their [0001] or [0001] crystalline planes, and are rotated by a non-zero angle with respect to their adjacent platelets along said stacking c-axis, wherein said zinc oxide microparticles are characterised by a specific surface area which is less than or equal to 4 m2/g. The invention also relates to a method for preparing the zinc oxide microparticles of the invention, comprising the steps of i) adding urea to an aqueous zinc salt solution； ii) heating the solution resulting from step (i)； iii) isolating the product resulting from step (ii)； and iv) submitting the isolated product from step (iii) to an annealing treatment. The invention further relates to a cosmetic product, paint, ink, paper, cardboard, textile, food, agricultural, home care, air conditioning, animal care, personal and work hygiene, contact lens, chromatography material, medical equipment, dermatological, lacquer, coating and/or plastic product containing the zinc oxide microparticles of the invention and to a biocide composition comprising the zinc oxide microparticles as defined above. Finally, the invention further relates to the use of the biocide composition of the invention for the elimination, inhibition of the growth, or inhibition of progeny of microorganisms.
