A composition for inhibiting metastasis and angiogenesis of hepatocellular carcinoma cells in a subject is disclosed. Particularly, the composition comprises (a) an effective amount of protein, the protein comprising the amino acid sequence of SEQ. ID NO:1 and having a trypsin inhibitor activity and (b) a pharmaceutically acceptable carrier.本發明揭露一種在個體中抑制肝癌細胞之移轉及血管新生的組合物。具體而言,該組合物包括(a)有效量之蛋白,該蛋白包括SEQ. ID NO:1之胺基酸序列及具有胰蛋白酶抑制因子之活性;及(b)藥學上可接受之載劑。