This invention relates to the production of isolated naive regulatory T cells by detecting the presence or absence of a panel of surface markers comprising CD3, CD4, CD25, CD45RA, CCR4 and CCR7 on the surface of cells in a population of peripheral blood mononuclear cells (PBMCs)； and separating cells on which the presence of the surface markers CD3, CD4, CD25, CD45RA, and CCR7 and the absence of the surface marker CCR4 is detected. Isolated populations of naive regulatory T cells and methods and kits for their production are provided.
