The automatic bearing device for living cell freezing and thawing includes a droplet changing area on one end and a sample bearing area communicating with the droplet changing area and arranged on the other end. The droplet changing area has a structure of a shallow dish for droplet change, and the bottom surface gradually inclines downwards from the free end to one end communicating with the sample bearing area. The sample bearing area has an elongated concave dish for containing frozen/thawed carriers. At least one part of the side of the droplet changing area is connected with the corresponding side of the sample bearing area. A sealer is arranged in the connection position, and a removable baffle is arranged above the connection position. The device realizes rapid and effective vitrification for living cell freezing and thawing.
