BIOPHARM GESELLSCHAFT ZUR BIOTECHNOLOGISCHEN ENTWICKLUNG VON PHARMAKA MBH
发明人:
POHL, JENS,PLOEGER, FRANK,KRUSE, MICHAEL
申请号：
ES06818642
公开号：
ES2382464T3
申请日:
2006.11.17
申请国别(地区):
ES
年份:
2012
代理人:
摘要：
Recombinant GDF-5-related protein with enhanced biological activity in which a) the amino acid in the position corresponding to methionine 453 (M453) of human wild-type GDF-5 (SEQ ID NO: 1) is alanine, valine or isoleucine, and / b) the amino acid in the position corresponding to methionine 456 (M456) of human wild type GDF-5 (SEQ ID NO: 1) is alanine, valine or isoleucine, and in which it comprises a sequence that matches one or more of the following generic amino acid formulas: a) CX1X2KX3LHVX4FX5X6X7GWDDWX8IAPLX9YEAX10HCX11GX12CX13FPX14RSHLEPTNHAX15 IQTLZTNSMX16PX17X18X19PX201X21CCVPX22X23LX24PISILX25X26DX27X28NNVVY X29X30YEX31MVVEX32CGCR b) CX1X2KX3LHVX4FX5X6X7GWDDWX8IAPLX9YEAX10HCX11GX12CX13FPX14RSH LEPTNHAX15 IQTLMNSZ2X16PX17X18X19PX201X21CCVPX22X23LX24PISILX25X26DX28NNVVYX29X30YEX31MVVEX32CGCR oc) CX1X2KX3LHVX4FX5X6X7GWDDWX8IAPLX9YEAX10HCX11GX12CX13FPX14RSH LEPTNHAX15 IQTLZ1NSZ2X16PX17X18X19PX201X21CCVPX22X23LPISILX25X26DX27X28NN VV YX29X30YEX31MVVEX32CGCR in which X1 indicates asparagine (N) or serine (S) X2 indicates arginine (R) or lysine (K) X3 indicates alanine (A), glutamine (Q), proline (P) or serine (S) X4 indicates asparagine (N) or aspartic acid (D) X5 indicates arginine (R) or lysine (K) X6 indicates aspartic acid (D) or glutamic acid (E) X7 indicates leucine (L) or methionine (M) X8 indicates isoleucine (I ) or valine (V) X9 indicates aspartic acid (D) or glutamic acid (E) X10 indicates histidine (H), phenylalanine (F) or tyrosine (Y) X11 indicates aspartic acid (D) or glutamic acid (E) X12 indicates leucine (L), methionine (M) or valine (V) X13 indicates aspartic acid (D) or glutamic acid (E) X14 indicates isoleucine (I) or leucine (L) X15 indicates isoleucine (I) or valine (V) X16 indicates alanine (A), asparagine (N) or aspartic acid (D) X17 indicates arginine (R), asparagine (N), aspartic acid (D), glutamic acid (E), glycine (G) or serine (S) X18 indicates alanine (A), asparagine (N), serine (S) or threonine (T) X19 indic a alanine (A), methio
