The pesticide protein has toxicity to lion diseases and insect pests, including but not limited to: tic6757, tic6757pl, tic7472, tic7472pl, A DNA structure containing a recombinant nucleic acid sequence is provided to encode one or more pesticide proteins. Provide plants, plant cells,The genetically modified seeds and some plants are resistant to reptile reproduction, contain recombinant nucleic acid sequences, and encode the existing insecticide proteins. Methods for detecting recombinant nucleic acid sequences in biological samples or proteins in the application are also provided, as well as methods for controlling diseases and insect pests of lions by using insecticide proteins tic6757, tic6757pl, tic7472 and tic7472pl. The recombinant nucleic acid of tic7473 and tic7473pl.requirement 1: a,it comprises a heterologous promoter operatively linked to a polynucleotide segment encoding a pesticidal protein or pesticidal fragment thereof, where: a) said pesticidal protein comprises the amino acid sequence of SEQ ID No. 4, SEQ ID No. 2, SEQ ID No. 6 , SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16 or SEQ ID No. 18； or b) said pesticidal protein comprises an amino acid sequence having at least 85%,Or 90%Or 95%Or 98%or 99% or about 100% amino acid sequence identity with SEQ ID No. 4, SEQ ID No. 2, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14 , SEQ ID No. 16 or SEQ ID No. 18； or c) said polynucleotide segment is hybridized under stringent hybridization conditions with a polynucleotide having the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 1, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15 and SEQ ID No. 17.Se describen proteínas pesticidas que presentan actividad tóxica contra especies de plagas de lepidópteros incluyen, de modo no taxativo, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473 y TIC7473PL. Se proporcionan construcciones de ADN que contienen una secuencia de ácido nucl
