Provided is a primary cell isolating apparatus that includes, sequentially adjacent, a slicing space, a working space, a picking space, and a sliding device mounted through the above spaces. The slicing space has a slicing device. The working space has a first gate, a heater, a rotating holder, a centrifuge, an open-close cap device, a tube rack, and a liquid transferring device. The first gate is adjacent to the slicing space. The heater is proximal to the first gate, and is sequentially adjacent to the rotating holder and the centrifuge. The open-close cap device is proximal to the first gate and is opposite the heater, and is sequentially adjacent to the tube rack and the liquid transferring device. The picking space has a second gate adjacent to the working space. Also provided is a method of using the primary cell isolating apparatus as abovementioned for saving manpower.
