GREGORY, Philip D.,HOLMES, Michael C.,MENDEL, Matthew C.,MENG, Xiangdong,PASCHON, David,REIK, Andreas,URNOV, Fyodor
申请号：
ES11840913
公开号：
ES2658691T3
申请日:
2011.11.17
申请国别(地区):
ES
年份:
2018
代理人:
摘要：
A zinc finger protein that binds to a target site in a PD1 gene, comprising five or six zinc finger recognition regions ordered from F1 to F5 or from F1 to F6, from end N to end C, and wherein the recognition regions comprise the following amino acid sequences: (i) F1: RSSALSR (SEQ ID NO: 23)； F2: RPLALKH (SEQ ID NO: 24)； F3: RNDHRKN (SEQ ID NO: 12)； F4: TRPVLKR (SEQ ID NO: 25)； and F5: DRSALAR (SEQ ID NO: 9), and wherein the zinc finger protein binds to the target site shown in SEQ ID NO: 60； (ii) F1: RPSTLHR (SEQ ID NO: 27)； F2: RSDELTR (SEQ ID NO: 28)； F3: RNNNLRT (SEQ ID NO: 29) or TNWHLRT (SEQ ID NO: 30) or RTPHLTL (SEQ ID NO: 31) or RSAQLAT (SEQ ID NO: 32) or RCTHLYL (SEQ ID NO: 33) or RPTQRYS (SEQ ID NO: 34) or RANHREC (SEQ ID NO: 35) or RMGRLST (SEQ ID NO: 39) or RANHRVC (SEQ ID NO: 42) or RSTHLLG (SEQ ID NO: 43)； F4: TRPVLKR (SEQ ID NO: 25)； and F5: DRSALAR (SEQ ID NO: 9) and wherein the zinc finger protein binds to the target site shown in SEQ ID NO: 60； (iii) F1: RPSTLHR (SEQ ID NO: 27)； F2: RSDELTR (SEQ ID NO: 28)； F3: RHSRLTT (SEQ ID NO: 40)； F4: TRPVLMR (SEQ ID NO: 41)； and F5: DRSALAR (SEQ ID NO: 9) and wherein the zinc finger protein binds to the target site shown in SEQ ID NO: 60； (iv) F1: RNAALTS (SEQ ID NO: 45)； F2: RSDELTR (SEQ ID NO: 28)； F3: RSCGLWS (SEQ ID NO: 44) or RPMHLTN (SEQ ID NO: 49) or RSPHLYH (SEQ ID NO: 50) or RLPALLS (SEQ ID NO: 53)； F4: TRPVLKR (SEQ ID NO: 25)； and F5: DRSALAR (SEQ ID NO: 9) and wherein the zinc finger protein binds to the target site shown in SEQ ID NO: 60； (v) F1: CNAALTR (SEQ ID NO: 46)； F2: RSDELTR (SEQ ID NO: 28)； F3: REEHRAT (SEQ ID NO: 47)； F4: TRPVLKR (SEQ ID NO: 25) and F5: DRSALAR (SEQ ID NO: 9) and wherein the zinc finger protein binds to the target site shown in SEQ ID NO: 60； (vi) F1: RNAALTR (SEQ ID NO: 45)； F2: RSDELTR (SEQ ID NO: 28)； F3: RCEALHH (SEQ ID NO: 51)； F4: TRPVLKR (SEQ ID NO: 25)； and F5: DRSAQAR (SEQ ID NO: 52) or DRSALAR (SEQ ID NO: 9) and wherein the zinc finger protein binds to the ta
