The invention discloses a method for preparing freeze-dried powder injection for treating a liver ascetic tumor by using aloe and lucid ganoderma. The freeze-dried powder injection is prepared from aloe powder and lucid ganoderma powder the method comprises the steps of: firstly preparing the aloe powder and the lucid ganoderma powder according to a proportion adding distilled water to form a mixed solution stewing for a night and then taking liquid supernatant for filtration and clarification adjusting the pH value to 6.3-6.5 by using hydrochloric acid, and sterilizing by heating and then cooling and adjusting the pH (Potential Of Hydrogen) value of the cooled liquid to 4.2-4.5, ultra-filtering through an ultra-filtration membrane, entrapping to obtain medicine-containing liquid with the molecular weight not greater than 10,000 Da, filling the medicine-containing liquid in ampoule bottles respectively, and drying by freezing so as to obtain the freeze-dried powder injection. The method for preparing freeze-dried powder injection for treating the liver ascetic tumor by using aloe and lucid ganoderma has the advantages of breaking through the traditional use range of the aloe which is only used as a product for expelling toxin, beautifying, relaxing the bowels and reducing lipid, mixing the aloe and the lucid ganoderma according to a certain proportion to form the freeze-dried powder injection by applying a modern medical means, and enabling the medical field to be widen to the aspect of treating the liver ascetic tumor. The experiment proves that the freeze-dried powder injection has outstanding treatment effects and no toxic and side effects, and the medical treatment field of the aloe is widened greatly.