PROBLEM TO BE SOLVED: To provide a method for obtaining site-specific recombination in a eukaryotic cell.SOLUTION: According to the present invention, a method comprises: providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site； and contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites. Here, the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB). The second recombination site is attB or attP, or the second recombination site is a pseudo attachment site. The recombinase is a Listeria monocytogenes A118 phage recombinase. When the first recombination attachment site is attB, the second recombination attachment site is attP. When the first recombination attachment site is attP, the second recombination attachment site is attB.SELECTED DRAWING: None【課題】真核生物において部位特異的組み換えを得る方法。【解決手段】第１の組換え部位及び第２の組換え部位を含む細胞を準備すること、原核生物のリコンビナーゼポリペプチドと共に第１及び第２の組換え部位を接触させ、組換え部位間で組換えを起こさせることを含み、リコンビナーゼポリペプチドが第１及び第２の組換え部位間での組換え反応を媒介でき、第１の組換え部位がファージゲノム組み換え付着部位（ａｔｔＰ）又は細菌ゲノム組み換え付着部位（ａｔｔＢ）であり、第２の組換え部位がａｔｔＢ又はａｔｔＰ、又は、第２の組換え部位が擬似付着部であり、リコンビナーゼが、リステリア・モノサイトゲネスＡ１１８ファージリコンビナーゼであり、第１の組換え付着部位がａｔｔＢであるとき、第２の組換え付着部位がａｔｔＰであり、第１の組換え付着部位がａｔｔＰであるとき、第２の組換え付着部位がａｔｔＢである方法。【選択図】なし
