#$%^&*AU2020100601A420200528.pdf#####Recombinant Broad-Spectrum Metarhizium and Production Method and Application thereof Abstract The present invention provides a recombinant broad-spectrum Metarhizium that down-regulates the expression of monoamine oxidase or does not express monoamine oxidase, or has a content of tryptamine therein higher than that in wild-type broad-spectrum Metarhizium, and the recombinant broad-spectrum Metarhizium is the strain per se, the progenies of the strain, the conidia produced by the strain, the mycelia produced by the strain, or any combination thereof. Knocking out monoamine oxidase gene from the recombinant broad-spectrum Metarhizium significantly increases the concentration of tryptamine in broad-spectrum Metarhizium robertsii, thereby significantly increasing the pesticidal efficiency thereof, and shortening the semi-lethal time LT50 of the wild-type broad-spectrum Metarhizium from 7.33± 0.445 days to 6.136 ± 0.488 days. The recombinant broad-spectrum Metarhizium is harmless to the environment, and has good biological safety and no toxicity to human beings.
