The disclosure relates to a firefly breeding device comprises at least one water tank with a first partition, a second partition, a drain hole and a water stop valve, wherein the first partition and the second partition divide the water tank into a plurality of space, and wherein the first partition is provided with a first hole disposed with a first filter, and the second partition is provided with a second hole disposed with a second filter, such that the plurality of spaces are connected. The drain hole is disposed at one side of the water tank with the water stop valve. The firefly breeding device of this invention is suitable for breeding fireflies at different growth stages, and the plurality of water tanks can be connected side by side and used to achieve the purpose of breeding fireflies artificially in a large-scale.本創作涉及一種螢火蟲養殖裝置,包括至少一個水槽,該水槽具有第一隔板、第二隔板、排水孔及止水閥,該第一隔板及該第二隔板將水槽分隔成多個空間,其中,第一隔板上設有第一孔洞,且該第一孔洞中設置有第一濾網,而第二隔板上設有第二孔洞,且該第二孔洞中設置有第二濾網,使該多個空間相連通;該排水孔設於該水槽的一側,且該止水閥設於該排水孔上。本創作的螢火蟲養殖裝置適用於養殖不同生長階段的螢火蟲,且多個水槽可並排連接使用,以達到以規模化的人工方式養殖螢火蟲的目的。