A dental floss holder and flosser is provided. The flosser provides a reusable dental device for cleaning between the user's teeth. The flosser comprises a casing, a pair of tines, and a floss advancement reel. A bundle of floss resides within the casing. A length of dental floss from the bundle of floss is fed through the casing and into the floss advancement reel. The length of dental floss is wrapped around the floss advancement reel. The length of dental floss is then strung taut along the pair of tines, forming a fresh segment of dental floss. The remaining length is routed and wrapped back to the floss advancement reel. A user can then rotate the floss advancement reel such that the segment of dental floss is replaced and ready for the next use.