CENTRE HOSPITALIER UNIVERSITAIRE GRENOBLE;UNIVERSITE JOSEPH FOURIER - GRENOBLE 1
发明人:
LE GOUELLEC, AUDREY,TOUSSAINT, BERTRAND,POLACK, BENOIT
申请号：
CA2857321
公开号：
CA2857321A1
申请日:
2012.12.12
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The invention is related to a process for obtaining an adapted strain of Pseudomonas, comprising the following steps: deleting the genes ExoS, ExoT, aroA and lasl in an initial Pseudomonas strain cultivated in a LB medium, progressively cultivating this strain in a chemically defined medium based on a glucose minimal medium supplemented with magnesium and calcium, wherein the adapted strain presents the same toxicity and secretion capacities than the initial strain, and its doubling time when cultivated in said chemically defined medium is less than 60 minutes. The present invention is also related to the adapted strain such as obtained, in particular wherein the adapted strain is furthermore treated to become 'killed but metabolically active'.
