An isolated, synthetic or recombinant nucleic acid comprising a nucleic acid sequence: (a) encoding a polypeptide having phospholipase activity, and (i) having at least 80%, 81%, 82%, 83%, 84 %, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or 100% of a sequence identity with SEQ ID NO: 177, and having a mutation encoding E41W, E41F or E41Y, (ii) hybridizes under conditions restrictive to the complement of a nucleic acid comprising SEQ IDNO: 177, and having a mutation encoding E41W, E41F, or E41Y, in which the restrictive conditions include a washing step comprising a 0.2XSSC wash at a temperature of about 65 ° C for about 15 minutes； (b) the nucleic acid sequence of (a) encoding a polypeptide having phospholipase activity but lacking: a signal sequence or proprotein sequence, or a homologous promoter sequence； (c) the nucleic acid of (a) or (b) encoding a polypeptide that has a phospholipase activity and additionally encoding an amino acid sequence, or the nucleic acid of (a) or (b) comprising a heterologous nucleotide sequence； (d) the nucleic acid of (c), in which the heterologous amino acid sequence comprises, or consists of, a sequence encoding a heterologous signal sequence, or a label or an epitope, or the heterologous nucleotide sequence comprises a heterologous promoter sequence； (e) the isolated, synthetic or recombinant nucleic acid of any one of (a) to (d), wherein the nucleotide residues at a cryptic transcriptional start site are modified to eliminate most or all of the production of a truncated transcript； (f) the isolated, synthetic or recombinant nucleic acid of (e), in which modifications of the nucleotide moieties at the cryptic transcriptional start site comprise an alteration at a ribosome binding site (RBS)； or (g) a nucleic acid sequence completely complementary to the nucleotide sequence of any of (a) to (f).Un ácido nucleico aislado, sintético o recombinante que comprende una secuencia de ácido nucleico:
