A multiple focal spot X-ray tube (100) comprising an electron source (105), which is adapted to generate an electron beam (106), an anode (110), which is arranged within the electron beam (106) and which comprises a first focal spot portion (120) and a second focal spot portion (130), whereby the second focal spot portion (130) is spatially separated from the first focal spot portion (120). The X-ray tube (100) further comprises a first electron beam manipulation unit (125), which is adapted to interact with the electron beam (106), when the electron beam (106) impinges onto the first focal spot portion (120), and a second electron beam manipulation unit (135), which is adapted to interact with the electron beam (106), when the electron beam (106) impinges onto the second focal spot portion (130). By assigning one electron beam manipulation unit (125, 135) to each of the focal spot portions (120, 130), a precise focusing of the X-ray beam can be realized individually for each focal spot of the X-ray tube (100). Preferably, the first and the second focal spot portions have a distance along the axis of a rotating anode.