RECOMBINANT PROTEINS CONTAINING ANTIGENIC EPITOPES OF PROTEINS CORE, SMALL ENVELOPE, NS2A AND PREM OF ZIKA VIRUS, AND COMPLEX ANTIGEN CONTAINING SPECIFIED PROTEINS WHICH ARE USED AS A COMPONENT OF THE IMMUNOENZYMOMETRIC TEST SYSTEM FOR THE IDENTIFICATION OF ANTIBODIES CLASSES G AND M TO ZIKA VIRUS
Federalnoe byudzhetnoe uchrezhdenie nauki "Gosudarstvennyj nauchnyj tsentr virusologii i biotekhnologii "Vektor" Federalnoj sluzhby po nadzoru v sfere zashchity prav potrebitelej i blagopoluchiya chel
发明人:
Pyankov Stepan Aleksandrovich (RU),Пьянков Степан Александрович (RU),Agafonov Aleksandr Petrovich (RU),Агафонов Александр Петрович (RU),Ruzhkova Alesya Aleksandrovna (RU),Ружкова Алеся Александровна (,Pyankov Stepan Aleksandrovich,Пьянков Степан Александрович,Agafonov Aleksandr Petrovich,Агафонов Александр Петрович,Ruzhkova Alesya Aleksandrovna,Ружкова Алеся Александровна,Maksimov Nikolaj Lvovich,М
申请号：
RU2017119301
公开号：
RU0002661085C1
申请日:
2017.06.01
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: invention relates to recombinant proteins containing antigenic epitopes of proteins Core, Small Envelope, NS2A and preM of the Zika virus, and a complex antigen containing these proteins. Obtained: recombinant protein (Z1), containing the antigenic epitopes of the Core protein of the Zika virus, characterized by the amino acid sequence (SEQ ID NO: 1) and used as the first component for the preparation of the complex antigen of the Zika virus； recombinant protein (Z2) containing the antigenic epitopes of the Small Envelope protein of the Zika virus, characterized by an amino acid sequence (SEQ ID NO: 2) and used as a second component to obtain the complex antigen of the Zika virus. Recombinant protein (Z3) containing the antigenic epitopes of the NS2A proteins of the Zika virus, characterized by an amino acid sequence (SEQ ID NO: 3), is used as the third component for the preparation of the complex antigen of the Zika virus, and the recombinant protein (Z4) containing the antigenic epitopes of the preM protein of the Zika virus, characterized by the amino acid sequence (SEQ ID NO: 4), it is used as the fourth component for the preparation of the complex antigen of the Zika virus. Complex antigen can be used as a component of an enzyme-linked immunosorbent assay system for the detection of G and M antibodies to the Zika virus and contains recombinant proteins Z1, Z2, Z3, Z4, mixed respectively in a ratio of 1:3:2:2. In the resulting and used proteins, there are no linear antigenic epitopes of other flaviviruses, and the sequences encoding these proteins do not have an antigenic cross with all known flaviviruses, which allows them to use more sensitive and accurate ELISA analysis.EFFECT: invention can be used in medical virology.5 cl, 4 tbl, 6 exИзобретение касается рекомбинантных белков, содержащих антигенные эпитопы белков Core, Small Envelope, NS2A и preM вируса Зика, и комплексного антигена, содержащего указанные белки. Получены: рек
