An image detection system for diagnosing a metabolic function and physiological status of a tissue or an organ having a fluorescent matter is provided. The image detection system includes a source emitting exciting light, a scan unit converting the exciting light into a scan of the exciting light, a light-guiding unit including an object lens, a splitter unit, and a detection unit. The splitter unit directs the scan of the exciting light to enter a tissue having a fluorescent matter such that the fluorescent matter in the tissue is excited by the scan of the exciting light and emits fluorescence light, whereby a layer of the tissue is scanned by the scan of the exciting light. The splitter unit directs the fluorescence light emitted from the tissue to the detection unit for processing so as to generate a fluorescent image of the layer of the tissue.
