PURPOSE: A composition containing probiotics for enhancing livestock immunity is provided to reduce livestock mortality and to reduce disease frequency. CONSTITUTION: A composition for enhancing livestock immunity contains probiotics of Escherichia fergusonii(1-3 weigh parts), Acinetobacter parvus(1-3 weight parts), Chryseobacterium indologenes(1-3 weight parts), and Bacillus liqueniformis(1-3 weight parts) as an active ingredient. The composition additionally contains 12-18 weight parts of lactose, 0.2-0.4 weight parts of vitamin A, 65-75 weight parts of fructo oligosaccharides, and 4-6 weight parts of chitosan. A method for preparing the composition comprises: a step of mixing the lactose, vitamin A, fructo oligosaccharides, and chitosan and sterilizing at 80-90°;C for 20-40 minutes; a step of adjusting pH concentration of the mixture using 1N-chloric acid; and a step of adding the strains.본 발명은 유효성분으로 에셰리키아 페르구소니(Escherichia fergusonii), 아시네토박터 파르부스(Acinetobacter parvus), 크리세오박테리움 인돌로제네스(Chryseobacterium indologenes) 및 바실러스 리케니포미스(Bacillus licheniformis)로 이루어진 생균을 함유하는 가축 면역 증진용 조성물, 상기 가축 면역 증진용 조성물의 제조방법 및 상기 조성물을 가축에게 급여하여 가축의 면역을 증진시키는 방법에 관한 것이다.