For genomic DNA of the test wheat derived from the nucleotide sequence of at least 15 bases of contiguous or its complementary sequence specific nucleotide sequence corresponding to the insertion sequence found in the abscisic acid 8 position hydroxylase gene of the wheat D genome By performing nucleic acid amplification using oligonucleotide primers of one or more, including, comprising detecting an insertion mutation in position hydroxylase gene, abscisic acid 8 of the test wheat abscisic acid 8 of the wheat D genome position a method for detecting a loss-of-function mutations in the hydroxylase gene.被験コムギ由来のゲノムDNAについて、コムギDゲノムのアブシジン酸8位水酸化酵素遺伝子中に見出された挿入配列に対応する特定の塩基配列又はその相補配列の連続した少なくとも15塩基の塩基配列を含む1種以上のオリゴヌクレオチドプライマーを用いて核酸増幅を行うことにより、コムギDゲノムのアブシジン酸8位水酸化酵素遺伝子中の挿入変異を検出することを含む、被験コムギのアブシジン酸8位水酸化酵素遺伝子の機能欠損変異を検出する方法。
