An antimicrobial traditional Chinese medicine composition, and a preparation method and a use thereof are provided, wherein the antimicrobial traditional Chinese medicine composition is made from raw materials comprising fructus perillae, patchouli and usnea. Through the reasonable matching application and synergistic effects of traditional Chinese medicines, the extracted composition has a broad-spectrum antimicrobial effect, and is suitable for being used as a natural preservative. Experiments show that for each microorganism of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger independently, the antimicrobial effect of the matched composition of the present invention is superior to the antimicrobial effect of single plants, the result where the total effect of the composition is better than sum of the effects of single plants is achieved, and the synergistic effect of antimicrobial effects among the plants is achieved.
