Disclosed is a method for eliciting the production of an affinity-matured antibody in a non-human subject, comprising steps of: (i) administering to the non-human subject a first antigen that binds to a germline antibody with greater affinity than to the affinity-matured antibody and then (ii) administering to the non-human subject a second antigen that binds to the affinity matured antibody with greater affinity than to the germline antibody, wherein the germline antibody and the affinity-matured antibody both bind to the first antigen and the second antigen, but wherein the germline antibody binds to the first antigen more tightly than it binds to the second antigen and the affinity-matured antibody binds to the second antigen more tightly than it binds to the first antigen.
