This invention relates to a method of producing a herbicide-resistant rice plant which comprises cultivating a callus comprising a cultured cell de dedifferentiated from a tissue of a rice plant in a plant tissue culture medium containing a herbicide added thereto, selecting and taking out a mutant of the callus having herbicide resistance proliferated in the medium containing the herbicide, transferring the mutant to a redifferentiation medium to redifferentiate into a young rice plant, cultivating the redifferentiated young rice plant, and selecting and collecting a plurality of herbicide-resistant seeds from the fructified rice plant. This invention also provides a herbicide-resistant rice plant or a seed of the same.