A method for producing a fungal extract having agricultural insect pest-killing ability by a new culture method of a fungal fungus having insect-killing ability of agricultural pests, To provide an effective insecticidal method with excellent immediate effect in a short time. Culturing a filamentous fungus in a solid medium to obtain a solid culture of the filamentous fungus； culturing the filamentous fungus in a liquid medium to obtain a liquid culture of the filamentous fungus； and a liquid culture of the filamentous fungus Separating the mycelium of the filamentous fungus, mixing the liquid culture from which the mycelium has been separated and the solid culture to obtain a mixed culture, and the filamentous form from the mixed culture A method for producing a fungal extract having the ability to kill agricultural pests, comprising a step of extracting a fungal spore solution, and an agricultural pest that causes the fungal extract obtained by the production method to act on agricultural pests or their habitats Insecticidal method.農業害虫の殺虫能を有する糸状菌の新たな培養方法による農業害虫殺虫能を有する菌体抽出物の製造方法と、当該製造方法によって製造された菌体抽出物に含まれる糸状菌による農業害虫の、短時間での即効性に優れた効果的な殺虫方法を提供すること。糸状菌を固体培地で培養し、前記糸状菌の固体培養物を得る工程と、前記糸状菌を液体培地で培養し、前記糸状菌の液体培養物を得る工程と、前記糸状菌の液体培養物から、当該糸状菌の菌糸体を分離する工程と、前記菌糸体が分離された前記液体培養物と前記固体培養物とを混合し、混合培養物を得る工程と、前記混合培養物から前記糸状菌の胞子液を抽出する工程を備える農業害虫殺虫能を有する菌体抽出物の製造方法及び当該製造方法で得られた菌体抽出物を農業害虫又はその生息地に対して作用させる農業害虫の殺虫方法。
