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MODIFIED BIOTIN-BINDING PROTEIN, FUSED PROTEINS ON ITS BASIS AND THEIR APPLICATION
专利权人:
ЧИЛДРЕН'С МЕДИКАЛ СЕНТЕР КОРПОРЕЙШН (US);ЧИЛДРЕНС МЕДИКАЛ СЕНТЕР КОРПОРЕЙШН (US)
发明人:
МОЛЛИ Ричард (US),ЛУ Инцзе (US),ЧЗАН Фань (US)
申请号:
RU2013154706/10
公开号:
RU2013154706A
申请日:
2012.05.11
申请国别(地区):
RU
年份:
2015
代理人:
摘要:
1. A soluble biotin-binding protein comprising the amino acid sequence of SEQ ID NO: 1 and any functional derivatives thereof. Biotin-binding protein according to claim 1, characterized in that the biotin-binding protein is obtained in soluble form at a level of at least 10 mg / l of culture medium in E. coli. 3. A biotin binding protein according to claim 1, characterized in that the biotin binding protein is a dimer. A biotin binding protein according to claim 1, characterized in that the biotin binding protein comprises a bacterial signal sequence at the N-terminus. Biotin-binding protein according to claim 4, characterized in that the bacterial signal sequence is SEQ ID No: 2.6. A biotin-binding protein according to claim 4, characterized in that the signal sequence is linked to a biotin-binding protein by a peptide linker. Biotin-binding protein according to claim 6, characterized in that the peptide linker comprises the amino acid sequence of SEQ ID NO: 8 or VSDP (SEQ ID NO: 9). A biotin binding protein according to claim 1, characterized in that the biotin binding protein includes a purification label at the C-terminus. The biotin-binding protein according to claim 8, characterized in that the purification label is selected from the group consisting of a histidine tag, a c-tag, a Halo tag, a Flag tag, and any combination thereof. Biotin-binding protein according to claim 9, characterized in that the histidine tag includes the amino acid sequence of SEQ ID NO: 10.11. A biotin-binding protein according to claim 9, wherein the purification label is linked to the biotin-binding protein via a peptide linker. Biotin-binding protein according to claim 11, characterized in that the peptide linker comprises an amino acid sequence1. Растворимый биотин-связывающий белок, включающий аминокислотную последовательность SEQ ID NO: 1 и любые его функциональные производные.2. Биотин-связывающий белок по п. 1, отличающийся тем, что биотин-связывающий белок получают в растворимо
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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