PROBLEM TO BE SOLVED: To provide a non-invasive estimation method of glucose and a non-invasive estimation device of glucose which enable the track of temperature-induced sugar decomposition in non-invasive estimation of glucose.SOLUTION: Owing to glucose metabolic change induction, glucose metabolism, and hemoglobin variant concentration change in a skin nutritional capillary, a localized reflectance optical probe which is adjusted at temperature different from skin temperature makes contact with skin. A time window for data collection which can minimize the impact of tissue-probe adaptability for a signal is selected. The change of a localized reflected light signal for various distances between a light source and a detector, various wavelengths, various degrees of contact time is measured from the skin contact of a probe for a given length of time. A function change in relation to the influence of heat stimulation for light absorption change by hemoglobin is calculated as a result of the influence of temperature for glucose metabolism. The function change in relation to the effect of heat stimulation for light attenuation change is calculated as a result of light scattering and blood flow change. The calibration relation between the combination of a function which is drawn from the signal of localized reflectance and glucose concentration is drawn. In order to predict a glucose concentration in a subject in subsequent time, measurement and the established calibration relation are used.
