LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
发明人:
申请号：
IL24186415
公开号：
IL241864D0
申请日:
2015.10.06
申请国别(地区):
IL
年份:
2015
代理人:
摘要：
Separating fibrinogen (I), factor XIII (II) and biological glue (III) from a solubilized plasma fraction (A), based on (I) and (II), and preparing lyophilized concentrates of these proteins, is new. Separating fibrinogen (I), factor XIII (II) and biological glue (III) from a solubilized plasma fraction (A), based on (I) and (II), and preparing lyophilized concentrates of these proteins comprises: (A) chromatographic purification by (i) loading a weakly basic anion exchanger, previously equilibrated with a basic buffer of predetermined ionic strength, with (A) such that (III) is retained and (ii) eluting (III) by increasing the ionic strength of the buffer； (B) separation of (II) from (I) by adding to some of the eluted (III) an agent (IV) that precipitates (II), then recovery of the remaining solution from the supernatant of purified (II)； and (C) diafiltration of the solutions of (I), (III) and redissolved (II), followed by lyophilization of the solution. An independent claim is included for lyophilized concentrates of (I), (II) and (III), prepared by the new method. ACTIVITY : Hemostatic； Anticoagulant； Thrombolytic； Vulnerary. No biological data given. MECHANISM OF ACTION : Fibrinogen is the precursor of fibrin clots.
