An isolator system 1 includes: a main isolator 3 in which an aseptic state is maintained and which is for performing an aseptic operation； an incubator 4 in which the aseptic state is maintained and which is connected to the main isolator 3 and is for culturing cells and the like； decontamination means 35 for decontaminating the inside of the main isolator 3； and a decontamination station 9 that decontaminates the inside of the incubator 4. The isolator system 1 further includes a blocking member 30 for sealing a connection port 13 from the outside, the port being provided in the main isolator 3 and being for connection with the incubator 4. When the inside of the main isolator 3 is decontaminated, the connection port 13 is sealed from the outside with the blocking member 30. The isolator system that can efficiently decontaminate the main isolator and the subisolator connected to this main isolator can be provided.
