Peter M. Rabinovich,Sherman M. Weissman,Marina E. Komarovskaya,Erkut Bahceci,Samuel Katz,Efim Golub
申请号：
US15008376
公开号：
US10155038B2
申请日:
2016.01.27
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Compositions and methods of making cells using RNA, and cells made using the disclosed compositions and methods are also provided. In exemplary embodiments, RNA is transfected into cells to effect a molecular, biological, physiological, or histological change in the cells. In preferred embodiments, the RNA is prepared in vitro, more preferably using a DNA template according to the provided compositions and methods. Methods for treating or inhibiting a disorder or disease such cancer are also provided. The methods can include, for example, locally or systemically administering to the host an effective amount of one or more RNAs or an effective amount of population of cells isolated from the subject or a syngeneic or histocompatible subject, contacted ex vivo with one or RNAs, and optionally expanded. The cells can be, for example, immune cells or stem cells.
