The invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns； b) providing a plurality of chromatography resin aliquots； c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns； and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns.
