HEO, GYO,SON, JI YOUNG,손지영,CHANG, KYUNG SOO,장경수,NA, DONG HEE,나동희
申请号：
KR1020110027131
公开号：
KR1020120108801A
申请日:
2011.03.25
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
PURPOSE: An anti-allergic composition containing fulvic acid is provided to suppress the secretion of various allergic materials and to ensure excellent anti-allergic effect.CONSTITUTION: An anti-allergic composition contains fulvic acid as an active ingredient. Fulvic acid suppresses the secretion of allergic materials from mast cells causing allergy. The mast cells are RBL-2H3 cells. A method for preparing the mast cell RBL-2H3 comprises: a step of culturing mast cells in a medium containing glutamine, antibiotics, and 10% FBS a step of collecting the mast cells by trypsin and culturing the mast cells with 200 ng/mL DNP-specific IgE a step of washing the mast cells with PIPES buffer solution(25 mM PIPES, pH 7.2, 119 mM NaCl, 5 mM KCl, 0.4 mM MgCl_2, 1 mM CaCl_2, 5.6 mM glucose, and 0.1% BSA) a step of pre-culturing the mast cells for 30 minutes and adding 2ng/ml of antigen.COPYRIGHT KIPO 2013본 발명은 풀빅산의 항알러지 효과에 관한 것으로서, 본 발명에 따른 실험을 통하여 알레르기를 유발하는 비만세포 RBL-2H3의 다양한 알레르기 유발물질의 분비를 풀빅산이 농도 의존적으로 억제함을 확인할 수 있었으며, 따라서 본 발명에 따른 풀빅산을 활성성분으로 포함하는 조성물은 우수한 항알러지 효과를 가지는 조성물임이다.
