The present invention relates to a nanofusion of liposome-nucleic acid fluorescence, an imaging system to use the nanofusion to diagnose a disease, a disease diagnosis method, and a method to prepare the nanofusion, wherein the nanofusion of liposome-nucleic acid fluorescence includes a silica sphere or a polystyrene sphere within a liposome having a surface to which a fluorescent material labeled-nucleic acid structure in a branched shape is attached. More specifically, the present invention relates to a technique used to prepare a nanofusion of liposome-nucleic acid fluorescence adhered to a high-performance diagnosis of a plurality of diseases, wherein the nanofusion is prepared by: preparing a nucleic acid structure in a Y-shape labeled by a fluorescent material； attaching the nucleic acid structure to a surface of a polystyrene or silica sphere to implement in the cells； and coating the surface of the sphere with a liposome bilayer through fusion technology. The nanofusion of liposome-nucleic acid fluorescence, in accordance to the present invention, can perform specific diagnosis by sensing a signal from the outside or inside of a cell membrane in accordance to a purpose； allow a high sensing diagnosis when targeting ribonucleic acid messenger and micro ribonucleic acid having absolutely low cellular concentration； and flexibly diagnosing carcinoma of which the diagnosis is difficult such as a triple negative breast cancer since various target materials expressed outside and inside a cell membrane can be targeted. Furthermore, the present invention is advantageous to simultaneously diagnose a plurality of cancers using various fluorescent materials depending on a disease to be diagnosed.본 발명은 형광체로 표지된 가지형태의 핵산구조체가 표면에 결합된 폴리스티렌 또는 실리카 구형체를 리포솜 내부에 포함하는 리포솜-핵산형광 나노 융합체, 상기 나노 융합체를 질병 진단에 활용하는 이미징 시스템, 질병 진단 방법, 및 상기 나노 융합체의 제조방법에 관한 것으로서, 더욱 구체적으로는 본 발명은 형광체로 표지된 Y자 형태의 핵산구조체를 제작하고 이를 세포 내에서 구현할 수 있도록 폴리스티렌 또는 실리카 구형체 표면에 부착시킨 후 융합기술(fusion technology)을 통해 리포솜
