A remote temperature measurement method for magnetic nano-particle based on paramagnetic property, comprises the following steps: (1) disposing a magnetic nano-sample at the position of an object to be measured； (2) applying n different exciting magnetic fields to the area where the magnetic nano-sample locates； (3) sampling the magnetization intensities of the magnetic nano-sample in different exciting magnetic fields, and computing the magnetic susceptibilities in different exciting magnetic fields based on the magnetization intensities； (4) constructing an equation set for the different exciting magnetic fields and the corresponding magnetic susceptibilities based on the langevin paramagnetic theorem； (5) solving the equation set to acquire a temperature T. The invention can detect the temperature of the object more precisely and rapidly, especially is suitable for detecting the biomolecule thermal motion.
