A system, method and media formulation for high-quality and large-scale micropropagation of graminaceous plants such as M.×giganteus and switchgrass have been developed and include callus induction, callus propagation, plantlet regeneration, shoot multiplication, shoot quality improvement and rooting, resulting in high plant survival in the greenhouse and in the field. The systems and methods described herein are theoretically capable of producing more than 700 billion plants from one single shoot in one year.
