Production method of the chimera animal which features the second versatile characteristic trunk cell where the first versatile characteristic trunk cell and the reproduction cell formation talent which become purpose of systematic establishment lack or decrease, or and that the aggregate of the embryo is transplanted to the landlord animal.系統樹立の目的となる第一の多能性幹細胞と、生殖細胞形成能が欠如又は低下した第二の多能性幹細胞と、胚との集合体を宿主動物に移植することを特徴とするキメラ動物の作製方法。