REPUBLIC OF KOREA(MANAGEMENT ; RURAL DEVELOPMENT ADMINISTRATION)
发明人:
HAN SANG MI,LEE KWANG GILL,PARK KWAN KYU,YOUNG MEE YOON
申请号：
NZ62619112
公开号：
NZ626191A
申请日:
2012.04.24
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is a method for the purification of bee venom on a mass scale. It comprises dissolving crude bee venom in a solvent to form a bee venom solution applying an adsorbent to the bee venom solution to form a mixture and removing the adsorbent through filtration to afford a pure bee venom solution. By the method of the present invention, crude bee venom collected from bee farms can be purified to a purity of 99% or higher on a mass scale, without inducing a change in the composition thereof, and thus the purified bee venom can be used as a material for medicines, cosmetics and foods. Particularly disclosed is a method for purifying bee venom on a mass scale, comprising: dissolving crude bee venom in water to form a bee venom solution, wherein the crude bee venom is used in an amount of from 0.02 to 0.1 % by weight, based on a total weight of the bee venom solution filtering the bee venom solution through a cellulose filter paper with a particle size of 20 to 30&mum under reduced pressure applying primary secondary amine (PSA) to the bee venom solution to form a mixture, wherein PSA is added in an amount of from 0.1 to 10 g per liter of the bee venom solution filtering the mixture through a cellulose filter paper with a particle size of 0.45 to 0.8&mum under vacuum filtration filtering the mixture through a cellulose filter paper with a pore size of 0.2&mum under vacuum filtration to afford a pure bee venom solution free of water-soluble foreign impurities and lyophilizing the pure bee venom solution at -20°C or lower to afford a pure bee venom solution having a purity of 99% or higher.
