Closed loop device (1) for medical use sterile Surgical Planning for the collection,The Transport and processing of prosthesis (P) and \/ or implantable Prosthetic materials or explanted and similarThat has at least a collection Chamber (2) provided with a Main Opening hermetically lockable (21, 21)For the introduction of the Prosthesis (P) and \/ or implantable Prosthetic materials or explanted and similar to analyze, to be contained in it, at least a containment Chamber (3),In a Solution containing sterile Way, filled by appropriate means through a Connector (25 ),For the chemical separation of bacteria that adhere to surfaces Animate or inanimate, such as Prosthetic and others, and \/ or bacterial biofilm,The containment Chamber (3) selectively communicates with the collection Chamber (2),So that the solution can be realized from the containment Chamber (3) The Collection Chamber (2),For the chemical separation of bacteria to the Prosthesis (P) and \/ or implantable Prosthetic materials or explanted and similar.That can be inserted into the collection Chamber (2), extraction means (51, 52, 53, 54, 55,56) of the solution for the chemical separation of bacteria from the collection Chamber (2),And a valve (4) interposed between Said Collection Chamber (2) and the containment Chamber (3),Adapted to allow the passage of such a solution for the chemical separation of bacteria from the containment Chamber (3) The Collection Chamber (2),The valve (4) is provided with a Shaft (41) which can be opened by breakingCharacterized by the solution to the chemical separation of bacteria and \/ or bacterial biofilm comprises a solution of dithiothreitol (DTT) and \/ or n-acetylcysteine and \/ or glutathione and \/ or other derivTwo thiol concentrations between 0.0001% and 100%, this Solution can dissolve the biofilm Matrix accordingly allowing the isolation and identification of bacteriaFor the valve with STEP (4) provides Selective in one direction, and the collection Chamber (2)