A culture container includes a tube having a first and a second opening respectively provided at two opposite ends thereof, and a cover attachable to and removable from the first opening of the tube, the cover including an inlet port for passage of a fluid substance and a receptacle for holding a substance consumable by an organism of interest, the receptacle being enclosed inside the tube and the inlet port communicating with a hollow interior of the tube when the cover is attached to the first opening of the tube. Moreover, a system and a method of transferring a cultured organism of interest include switching the covers between two culture containers.