The present invention discloses a packaging box comprising separation plates, wherein the separation plate comprises an upper separation film, a lower separation film and a frame, the upper separation film and the lower separation film are fixed on the frame, and a pre-set distance is provided between the two films. During packaging, a fruit is packaged by snapping with the separation plates from top and bottom, or the fruit is packaged by snapping directly with the upper separation film and the lower separation film of the same separation plate from top and bottom. By packaging the fruit with the upper separation film and the lower separation film which have a certain elasticity and a tensile force, the fruit can be fixed via elastic deformation, the fruits in the same layer can be prevented from colliding against each other, and a certain distance is provided between separation plates and between the upper separation film and the lower separation film so as to form a buffer area, which prevents the fruits i
