The present invention relates to a composition containing growth factors and the method of preparing the composition. The method includes the following steps: obtaining autologous blood isolating a composition of the blood from the autologous blood preparing a first cell culture medium by using the composition of the blood culturing at least one autologous cells for 1 to 3 days, allowing the autologous cells to secrete a plurality of specific growth factors and forming a second cell culture medium isolating the second cell culture medium preparing the composition containing growth factors by using the second cell culture medium wherein the growth factors includes vascular endothelial growth factor, hepatocyte growth factor, and the growth factors further includes keratinocyte growth factor.本發明係關於一種生長因子製劑及其生產方法,包含以下步驟:取得一自體血液;從自體血液中分離出一血液組成物;以血液組成物製備一第一細胞培養液;以第一細胞培養液培養至少一自體細胞1至3天,使自體細胞分泌複數種生長因子至第一細胞培養液中,形成一第二細胞培養液;取出第二細胞培養液;以及將第二細胞培養液,製備成一生長因子製劑,生長因子至少包含血管內皮生長因子及肝細胞生長因子,或更進一步包含角質生長因子。
