YOON, IN JOONGKR,윤인중,CHOI, HWAN WONKR,최환원,YOO, SUNG SIKKR,유성식,LEE, SEUNG CHULKR,이승철,JANG, JUN HYUKKR,장준혁,KIM, SEA HWANKR,김세환,SHIM, YOUNG JUNGKR,심영정,AHN, YOUNG HOONKR,안영훈,JANG, HYUNG KWANKR,장형관,CHA, SE
申请号：
KR1020160005910
公开号：
KR1020170086274A
申请日:
2016.01.18
申请国别(地区):
KR
年份:
2017
代理人:
摘要：
The present invention relates to a live-mixed lyophilized vaccine of duck hepatitis A virus type 1 and type 3 and a preparation method thereof and, more specifically, relates to a live-mixed lyophilized vaccine of duck hepatitis A virus type 1 and type 3 which is obtained by mixing a high solution bacteria (HSB) strain which is a type 1 vaccine strain and an AP-04203P100 strain which is a type 3 vaccine strain of duck hepatitis A virus, which is domestically prevalent, with a lyophilization protective agent and then lyophilizing the mixture. The present invention exhibits an excellent protection rate against both domestically prevalent duck hepatitis type 1 and type 3 field strains by mixing the type 1 vaccine strain (HSB strain) and the type 3 vaccine strain (AP-04203P100 strain) of the duck hepatitis A virus at an optimum ratio. In addition, since a specific lyophilization protective agent is added, an excellent lyophilization recovery rate is achieved and the shelf life is greatly improved such that virus titer can be maintained for a long period of time under normal refrigeration conditions rather than freezing conditions. As a result, the present invention has an excellent protection effect against all the duck hepatitis viruses which are domestically prevalent and is easily distributed in the market and thus can be very useful as a comprehensive vaccine for the prevention of duck hepatitis, which is convenient for long-term storage in first-line duck farms.COPYRIGHT KIPO 2017본 발명은 오리 간염 A 바이러스의 1형과 3형의 생혼합 건조백신 및 그 제조방법에 관한 것으로, 더욱 상세하게는 국내에서 유행하는 오리 간염 A 바이러스의 1형 백신주인 HSB주(duck hepatitis A virus HSB strain)와 3형 백신주인 AP-04203P100주(duck hepatitis A virus AP-04203P100 strain)를 동결건조보호제와 혼합하여 동결건조한, 오리 간염 A 바이러스 1형과 3형의 생혼합 건조백신에 관한 것이다. 본 발명은 오리 간염 A 바이러스 1형 백신주(HSB주)와 3형 백신주(AP-04203P100주)를 최적의 비율로 혼합하여 국내에서 유행하는 오리 간염 1형과 3형 야외주에 대해 모두 우수한 방어율을 나타낸다. 또한, 특정한 동결건조보호제를 첨가하여 동결건조회수율이 뛰어날 뿐만 아니라, 냉동이 아닌 일반 냉장조건에서도 장기간 바이러스 역가를 유지할 수 있는 등 유통기한이 매우 개선되었다. 따라서, 본 발명은
