A dental implant positioning and guiding assembly is disclosed in the present invention. The dental implant positioning and guiding assembly includes a positioning rod, a convex element, a concave element, a connector and a plurality of guiding elements. The positioning rod is utilized to be inserted into a drilled hole in a patient's oral model. The convex element has a through hole for holding the positioning rod. The concave element is positionally assemble with the convex element. The connector is fixed to one side of the concave element and has a contact surface with a surface contour matching an oral contour of the patient for engaging with the oral contour. Each of the guiding elements is positionally assembled with the concave element and has an open with a curve guiding surface for guiding the surgery operator to drill the at least one implant hole.