1. A method for determining the binding constant of the compound of interest from proteins, comprising the following steps: a) adding a high affinity compound as a two-chamber system in which the two chambers are separated by a semipermeable membrane which is permeable to a compound of interest, and determining the amount of high-affinity compound of interest in one of the chambers, after equilibrium was reached distribution, b) adding the compound down into one of the chambers, where no compound downward mo a permeate through the membrane, and determining the coefficient of distribution of the compound of interest for down-the connection after been reached equilibrium distribution, c) adding a non-specific protein to another chamber, where a non-specific protein can not penetrate through the membrane, and determining a compound of the distribution coefficient of interest for non-specific protein in the presence of a compound of the down after the distribution equilibrium has been reached, and d) determining the coupling constant Nia test compound with a distribution coefficient of the steps b) and c) .2. The method of claim 1, wherein the filter is selective by size membranoy.3. The method of claim 1 wherein lowering compound is PVP or tsiklodekstrin.4. The method of claim 1 wherein the compound is a downward PVP25.5. The method of claim 1, wherein the protein is a protein plazmy.6. A method according to claim 5, wherein the plasma protein is a serum albumin.7. A method according to any one of claims 1-6, wherein steps a), b) and c) are performed in parallel.1. Способ определения константы связывания соединения, представляющего интерес, с белками, включающий следующие этапы:а) добавление высокоаффинного соединения в двухкамерную систему, в которой две камеры разделены полупроницаемой мембраной, которая проницаема для соединения, представляющего интерес, иопределение количества высокоаффинного соединения, представляющего интерес, в одной из камер после того, как был
