ВАЛЬТЕР Вольфганг (DE),ШЛАГ Петер М. (DE),КОБЕЛЬТ Деннис (DE),ШМИДТ Мануэль (DE)
申请号：
RU2013115266/10
公开号：
RU2013115266A
申请日:
2011.09.08
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. Application gantelepodobnoy linear covalently closed structure with double-stranded DNA substrate and the single-stranded loops located at both ends of the bases, where the base complementary to the deoxyribonucleic acid comprises circular DNA chain promoter sequence, coding sequence and termination signal, which is administered by needleless injection for treating cancer. 2. Use according to claim. 1, wherein the promoter sequence is operative in eukaryotic cells and cheloveka.3 body. Use according to claim. 1 or 2, wherein the DNA construct encodes an immunomodulating agents and / or marker kletok.4 surface. Use according to claim. 3, wherein the immunomodulatory agents are selected from the group consisting of antibodies, hormones, cytokines or other biologically active veschestva.5. Use according to claim. 4, wherein the cytokines are selected from the group consisting of TNF-α, interleukin-7, colony stimulating factor, granulocyte-macrophage, CD40L / CD154 and B7.1 / CD806. Use according to claim 1, 2, 4 or 5, wherein the DNA construct is introduced simultaneously or sequentially with a chemotherapeutic agentom.7. Use according to claim. 6, where the chemotherapeutic agent is selected from the group consisting of antibodies, alkylating agents, platinum analogs, intercalating agents, antibiotics, suppressors mitosis taxanes suppressor topoisomerases, antimetabolites and / or L-asparaginase, hydroxycarbamide, mitotane and / or amanitiny.8. Use according to claim. 7, where the suppressor is vindezin.9 mitosis. Use according to claim 1, 2,1. Применение гантелеподобной линейной, ковалентно замкнутой конструкции ДНК с двухцепочечной основой и одноцепочечными петлями, расположенными на обоих концах основы, где основа комплементарных дезоксирибонуклеиновых кислот кольцевой цепочки ДНК включает промоторную последовательность, кодирующую последовательность и сигнал терминации, которая вводится путем безыгольной инъекции для лечения рака.2. Применение в соответствии с
