The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha- functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega- functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha- functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl- CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree.La présente invention concerne lutilisation de micro-organismes pour préparer des produits chimiques fonctionnalisés en alpha et des carburants, (par exemple des acides carboxyliques, alcools, hydrocarbures, amines fonctionnalisés en alpha ainsi que leurs dérivés fonctionnalisés en bêta et en oméga), en utilisant une voie dallongement de chaine carbonée itérative qui utilise des motifs dextension fonctionnalisés. Les enzymes centrales dans ladite voie comprennent les thiolase, déshydrogénase, déshydratase et réductase. Les thiolases natives ou modifiées catalysent la condensation damorces dacyl-CoA soit non substitu
