Inside two these chains DNA inside the cell, it designates that nuclear acid construction ones which can introduce mutation into the target territory are offered as purpose, in order to achieve this purpose, 1 or plural bases are deficient at the time of base arranging of the inner sense chain side territory or the anti sense chain side territory of the target territory which exists in two these chains DNA inside the cell, consist of the base arrangement which substitution, it is inserted or is added, or, one chain DNA and the description above where homology of base arrangement of the aforementioned sense chain side territory or the aforementioned anti sense chain side territory is 90% or moreBase arrangement of portion of one chain DNA complimentary base arrangement is included, nuclear acid construction ones which possess with the one chain nuclear acid which annealing is done are offered to the aforementioned one chain DNA.細胞内の二本鎖DNAのうち、標的領域に変異を導入することができる核酸構築物を提供することを目的とし、この目的を達成するために、細胞内の二本鎖DNAに存在する標的領域のうちセンス鎖側領域又はアンチセンス鎖側領域の塩基配列において、1又は複数個の塩基が欠失、置換、挿入又は付加された塩基配列からなり、前記センス鎖側領域又は前記アンチセンス鎖側領域の塩基配列との相同性が90%以上である一本鎖DNAと、前記一本鎖DNAの一部の塩基配列と相補的な塩基配列を含み、前記一本鎖DNAにアニーリングした一本鎖核酸とを有する核酸構築物を提供する。
