Method for obtaining dna constructs, isolated dna fragment, molecular vector, genetically modified host cell, modified 3-domain cry toxin, method for obtaining modified 3-domain cry toxin, composition, method for suppressing resistance, method for suppressing resistance of the chosen resistant insects
UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO;ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
发明人:
LILIANA PARDO-LOPEZ,MARIO SOBERON-CHAVEZ,MARIA ALEJANDRA BRAVO-DE LA PARRA,ELLIOT BRUCE TABASHNIK
申请号：
BRPI0721721
公开号：
BRPI0721721A2
申请日:
2007.06.08
申请国别(地区):
BR
年份:
2013
代理人:
摘要：
METHOD TO OBTAIN DNA CONSTRUCTIONS, ISOLATED DNA FRAGMENT, MOLECULAR VECTOR, GENETICALLY MODIFIED HOST STONE CELL, CRY 3 DOMAIN TOXIN METHOD, METHOD MODIFIED METHOD TOO CRY TOXINE, MODIFIED METHOD TOGETHER RESISTANCE OF CHOSEN RESISTANT INSECTS. The present invention provides a method for obtaining DNA constructs encoding 3-domain Cry toxins (also called Cry toxins, Bt toxins or 5-endotoxins) devoid of the α helix. These DNA constructs have been modified to encode proteins capable of killing insects that exhibit resistance to the corresponding unmodified Cry toxins. The DNA constructs of the modified 3-domain Cry toxin genes and the modified 3-domain Cry proteins are presented together with the methods, molecular vectors and host cells containing these constructs, as well as recombinant methods for producing the Cry 3 toxin toxins. 3 modified domains. In addition, formulations containing modified 3-domain Cry Toxins are described in this invention. Insect resistance to unmodified Cry toxins is due to lower toxin binding to receptors in the insect gut. The present invention provides the expression of modified 3-domain Cry toxin constructs, methods of expression of these modified 3-domain cry toxins, transformed microorganisms and transgenic plants with constructs expressing modified 3-domain Cry toxins, and methods for suppress insect pest resistance to unmodified Cry toxins.MÉTODO PARA OBTER CONSTRUÇÕES DE DNA, FRAGMENTO DE DNA ISOLADO,VETOR MOLECULAR, CELULA HOSPEDEIRA GENETICAMENTE MODIFICADA, TOXINA CRY DE 3 DOMINIOS MODIFICADA, METODO PARA OBTER TOXINA CRY DE 3 DOMÍNIOS MODIFICADA, COMPOSIÇçO, MÉTODO PARA OBTER UMA PLANTA TRANSGÊNICA, METODO PARA SUPRIMIR A RESISTÊNCIA DOS INSETOS RESISTENTES ESCOLHIDOS.A presente invenção apresenta um método para obtenção de construções de DNA que codificam as toxinas Cry de 3 domínios (também chamadas toxinas Cry, toxinas Bt ou 5-endotoxinas) desprovidas da hélice a-l. Estas construções de DNA foram modificadas para codific
