An apparatus for non-invasive glucose monitoring includes a first light source for emitting at least one ray of first light a first beam splitter with a focusing function a set of photo detectors for measuring optical rotatory distribution (ORD) information and absorption energy information of the first light reflected from the eyeball and transmitted through the first beam splitter to the set of photo detectors, and the first light emitted from the first light source being transmitted to the set of photo detectors by the first beam splitter and the eyeball to form an optical path a processing unit receiving and processing the ORD information and the absorption energy information to obtain glucose information and an eye positioning device including a second beam splitter disposed on the optical path between the first beam splitter and the eyeball and a camera for receiving image information transmitted from the second beam splitter.
